General Cell Based Assays
Abcam HRV 3C Protease Inhibitor Assay Kit (Colorimetric)
HRV 3C Protease Inhibitor Assay Kit (Colorimetric)
The product is subject to the following: Abcam Restricted Use Statement
Arbor Assays Glutathione Colorimetric Detection Kit (4 Plate)
The DetectX Glutathione (GSH) Detection Assay Kits allow for convenient, rapid, and easy quantitation of both GSH and GSSG for an accurate measurement of a cell's oxidative status in a variety of sample types.
Arbor Assays Creatinine Serum Low Sample Volume Kit (384-well Plate)
The DetectX Low Volume Serum Creatinine kit quantitatively measures creatinine present in serum samples using a 384-well plate format to minimize sample usage.
TRIBIOSCIENCE INC NADP/NADPH Quantification Colorimetric Assay 100 Tests
Tribioscience NADP/NADPH Quantification Colorimetric Assay is based on a dehydrogenase coupled reaction convert chromophore to a colored formazan, which can be measured at OD 460 nm. The generated signal is proportional to the content of NADPH. This assay specifically detects NADP and NADPH from samples, not detect NAD/NADH. The kit provides the easiest and most accurate approach to measure NADPH from a variety of samples. Applications - Specifically detect NADP/NADPH from cell and biological tissues.
TRIBIOSCIENCE INC Glycerol Colorimetric Assay 100 Tests
Tribioscience’s Glycerol Colorimetric Assay kit is a robust, simple, and high throughput method based on coupled enzyme reactions. Glycerol is phosphorylated and oxidized to generate hydrogen peroxide, which is catalyzed and reacts with the probe measured by the colorimetric method at OD = 570 nm.
TRIBIOSCIENCE INC Mitochondrial Complex III Activity Colorimetric Assay 100 Tests
The activity of Complex III is directly measured through the increase of reduction of cytochrome c at absorbance of 550nm. This kit uses cytochrome c as substrate. The reduced cytochrome c is determined by recording the change in absorbance (A550). Applications Complex III Activity.
TRIBIOSCIENCE INC Cytochrome C Oxidase Activity Assay 100 Tests
The colorimetric assay in this kit is based on the observation of the decrease in absorbance at 550 nm of ferrocytochrome c caused by its oxidation to ferricytochrome c by cytochrome c oxidase. It is suitable for the detection of mitochondrial outer membrane integrity and for the detection of mitochondria in subcellular fractions.
TRIBIOSCIENCE INC Lactate Colorimetric Assay 100 Tests
Tribioscience’s Lactate Assay Kit is a simple and fast colorimetric assay that measures the total amount of L-Lactate in biological samples. Lactate is oxidized by lactate oxidase to pyruvate and hydrogen peroxide. Horseradish peroxidase (HRP) catalyzes the reaction between hydrogen peroxide and the specific probe to yield a final product measured at OD570 nm.
TRIBIOSCIENCE INC Fast Glucose Oxidase Activity Assay (Colorimetric/Fluorometric) 200 Assays
Tribioscience’s Fast Glucose Oxidase Activity Assay uses a single working reagent that combines the glucose oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 570nm or fluorescence intensity at λ ex/em = 530/585 nm is directly proportional to glucose oxidase activity in the sample. The fluorometric assay is more sensitive than the colorimetric assay. Applications - Direct Assay - Glucose oxidase activity in serum, plasma, urine, and other bio-samples. Key Features - Flexible - Suitable for colorimetric and fluorometric methods. Accurate - Use 50μL samples. Detection ranges 0.1-50 μM in 96-well plate for colorimetric assay and 0.002-10 μM for fluorometric assay. Simple and High-Throughput - One-step procedure. Load-Incubate-Read. The kit can be used for a robust method. Time Saving - takes less than 30 minutes.
TRIBIOSCIENCE INC HDAC Colorimetric Assay Kit 100 Tests
The Tribioscience HDAC Colorimetric Assay Kit provides a convenient, sensitive, and simple approach to quantify HDAC activity from various sources of enzyme using a colorimetric substrate. In HDAC assay, an acetylated substrate is incubated with HDAC-containing samples. Deacetylation of substrate sensitizes it in presence of HDAC developer, generates the colorimetric substrate, which can be detected at OD395nm. The substrate included in the kit is cell-permeable, and the assay can measure HDAC activity directly in cell culture in a 96-well plate without a time-consuming cell extraction step. The kit also can be used for HTS of HDAC inhibitors with extracts or purified enzymes.
Eurofins Discoverx PathHunter eXpress mCXCR5 CHO-K1 β-Arrestin GPCR Assay, 2-Plate Kit. (93-0474E2MCP0M)
The PathHunter mCXCR5 eXpress kit provides an easy-to-use cell based assay to study drug candidates (small molecules or biologics) targeting GPCRs. This kit assesses ligand (e.g. CXCL13/BCA-1/BLC) based activation of mCXCR5 GPCR activity via detection of β-arrestin recruitment. The included cell line overexpresses ProLink-tagged mCXCR5 and EA-tagged β-Arrestin-2. Activation of mCXCR5 stimulates the recruitment of β-Arrestin-2 and produces EFC signal. eXpress kits are a convenient, ready-to-use format which contains all materials needed to run the assay, including single-use vials of cryopreserved cells, cell plating media, detection reagent, and assay plates. Please note that this product expresses the Mouse version of this protein. For detailed product information, User Manual and Datasheet, visit www.discoverx.com/express.
Meso Scale Discovery RPLEX HUM Kallikrein3/PSA ASY
The R-PLEX Human Kallikrein 3 (PSA) Assay from Meso Scale Diagnostics is an electrochemiluminescent immunoassay for the quantitative measurement of total prostate-specific antigen (PSA) in human serum plasma and urine This assay utilizes MSD s proprietary SECTOR Imager 6000 for high sensitivity and a broad dynamic range The assay has a lower limit of detection of 0 01 ng/mL PSA and an upper limit of 100 ng/mL PSA The assay requires 25 L of sample and can be completed in under 3 hours This assay provides a sensitive accurate method for measuring PSA levels
Meso Scale Discovery U-PLEX Human TRAIL ASY
The U-PLEX Human TRAIL Assay K151XTK from Meso Scale Discovery is a multiplex immunoassay kit for the quantitative measurement of TRAIL in human serum plasma and cell culture supernatants The assay utilizes MSD s electrochemiluminescent detection technology and is provided in a 96-well plate format The kit includes a plate coated with capture antibodies detection antibodies calibrators and diluent The assay has a typical sensitivity of 0 3 pg/mL and an upper limit of quantification of 2500 pg/mL for TRAIL The total assay time is approximately 3 5 hours This product can aid in the quantitative determination of TRAIL levels in human samples